Journal: Animals : an Open Access Journal from MDPI
Article Title: From Gene to Protein: Unraveling the Reproductive Blueprint of Male Grey Squirrels via Nerve Growth Factor (NGF) and Cognate Receptors
doi: 10.3390/ani14223318
Figure Lengend Snippet: Antisera characteristics. The table shows the antisera name, the species in which the antibody is raised, the working solution, and the antibody producer.
Article Snippet: The transferred proteins were probed with different antibodies, each one incubated overnight at 4 °C in separate western blots with rabbit anti-NGF (1:500; n . MA5-32067 Invitrogen, Waltham, MA, USA), rabbit anti-p75 (1:1000; ab52987, Abcam, Cambridge, UK), rabbit anti-pan-TRK (1:1000; n . ab109010 Abcam), and mouse anti-Actin (1:1000; clone AC-40, Merk Sigma-Aldrich, Darmstadt, Germany) antibodies.
Techniques: Recombinant, Plasmid Preparation